Tg(Myh6/tetO-Tnnc1*L48Q)2Jmol
Transgene Detail

Summary

• Symbol: Tg(Myh6/tetO-Tnnc1*L48Q)2Jmol
• Name: transgene insertion 2, Jeffery Molkentin
• MGI ID: MGI:5897318
• Transgene: Tg(Myh6/tetO-Tnnc1*L48Q)2Jmol Location: unknown
• Alliance: Tg(Myh6/tetO-Tnnc1*L48Q)2Jmol page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Myh6/tetO-Tnnc1*L48Q)2Jmol expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Tnnc1 (290561)

  Tnnc1

  (MGI:98779)

  contains L48Q substitution

• Mutation details: A cDNA encoding rat TNNC1 (troponin C, cardiac/slow skeletal) carrying an L48Q (CTG->CAG) mutation and a FLAG epitope tag was placed under the regulatory control of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter and the tetracycline responsive element (tetO). Translation is terminated by a human growth hormone polyadenylation signal. When active, the transgene elicits ~40% incorporation of the mutant cardiac troponin C into the cardiac muscle sarcomere. (J:234490)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Transgenic mice exhibit 5% to 10% replacement at baseline in the absence of tTA transgene.

References

• Original: J:234490 Davis J, et al., A Tension-Based Model Distinguishes Hypertrophic versus Dilated Cardiomyopathy. Cell. 2016 May 19;165(5):1147-59
• All: 1 reference(s)