Igsf11^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Igsf11^{tm1d(KOMP)Wtsi}
• Name: immunoglobulin superfamily, member 11; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5897622
• Gene: Igsf11 Location: Chr16:38713033-38847521 bp, + strand Genetic Position: Chr16, 26.87 cM
• Alliance: Igsf11^{tm1d(KOMP)Wtsi} page
• IMPC: Igsf11 gene page

Mutation origin

• Mutant Cell Line: EPD0297_2_A07
• Germline Transmission: Earliest citation of germline transmission: J:234439
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 38828713 of Chromosome 16 upstream of the critical exons 3 through 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 3 through 5 at position 38829597. The critical exons 3 through 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis confirmed the absence of protein expression in the brain. (J:234439)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igsf11 Mutation: 33 strains or lines available

Notes

Cell line EPD0297_2_A07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:234439

References

• Original: J:234439 Jang S, et al., Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity. Nat Neurosci. 2016 Jan;19(1):84-93
• All: 2 reference(s)