Ube2w^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ube2w^{tm1d(EUCOMM)Wtsi}
• Name: ubiquitin-conjugating enzyme E2W (putative); targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5897638
• Gene: Ube2w Location: Chr1:16611012-16689676 bp, - strand Genetic Position: Chr1, 5.1 cM, cytoband A3
• Alliance: Ube2w^{tm1d(EUCOMM)Wtsi} page
• IMPC: Ube2w gene page

Mutation origin

• Mutant Cell Lines: EPD0156_4_C07, EPD0156_4_C08
• Germline Transmission: Earliest citation of germline transmission: J:230053
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 16667939 of Chromosome 1 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 16668723. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis confirmed the absence of protein expression in brain, spinal cord, heart, lung, kidney, liver, spleen, testis, pancreas and thymus. All three isoforms are affected by this allele. (J:230053)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube2w Mutation: 12 strains or lines available

References

• Original: J:230053 Wang B, et al., Loss of the Ubiquitin-conjugating Enzyme UBE2W Results in Susceptibility to Early Postnatal Lethality and Defects in Skin, Immune, and Male Reproductive Systems. J Biol Chem. 2016 Feb 5;291(6):3030-42
• All: 2 reference(s)