Cdh23tm1.1Kjn
Targeted Allele Detail
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Symbol: |
Cdh23tm1.1Kjn |
Name: |
cadherin related 23 (otocadherin); targeted mutation 1.1, Kenneth R Johnson |
MGI ID: |
MGI:5897899 |
Synonyms: |
c.753A, Cdh23c.753A |
Gene: |
Cdh23 Location: Chr10:60138527-60532269 bp, - strand Genetic Position: Chr10, 30.11 cM
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Alliance: |
Cdh23tm1.1Kjn page
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Allele Type: |
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Targeted (Not Specified) |
Mutations: |
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Insertion, Single point mutation
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Mutation details: Chr10: 60,527,162 bp 60,536,889 bp (GRCm38), which contains the c.753A point mutation but is otherwise identical in sequence to the 129S1/SvImJ sequence, was taken from a C57BL/6J-derived BAC, a loxP-flanked PGK-Neo cassette was inserted 178 bp downstream of the c.753 nucleotide, homology arms were added to correctly target this sequence to replace the endogenous coding sequence, resulting in a G to A point substitution with a loxP-flanked PGK-Neo cassette. Subsequent breeding to a cre deleter strain excised the selection cassette and generated this allele. Cochlear transcripts show increased exon skipping as a result of this point mutation, and this is predicted to delete part of the 2nd and 3rd ectodomains.
(J:241137)
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Inheritance: |
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Recessive |
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:241137 Johnson KR, et al., Effects of Cdh23 single nucleotide substitutions on age-related hearing loss in C57BL/6 and 129S1/Sv mice and comparisons with congenic strains. Sci Rep. 2017 Mar 13;7:44450 |
All: |
1 reference(s) |
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