Cdh23^tm2.1Kjn
Targeted Allele Detail

Summary

• Symbol: Cdh23^tm2.1Kjn
• Name: cadherin related 23 (otocadherin); targeted mutation 2.1, Kenneth R Johnson
• MGI ID: MGI:5897995
• Synonyms: c.735G, Cdh23^c.735G
• Gene: Cdh23 Location: Chr10:60138527-60532269 bp, - strand Genetic Position: Chr10, 30.11 cM
• Alliance: Cdh23^tm2.1Kjn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241137
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: Chr10: 60,527,162 bp 60,536,889 bp (GRCm38), which contains the functional c.753G variant, was taken from a 129S1-derived BAC, a loxP-flanked PGK-Neo cassette was inserted 178 bp downstream of the c.753 nucleotide, homology arms were added to correctly target this sequence to replace the endogenous coding sequence via homologous recombination in C57BL/6N-derived ES cells, resulting in a A to G point mutation, which effectively reverts the ahl allele to wild-type, with a downstream loxP-flanked PGK-Neo cassette. Subsequent breeding to a cre deleter strain excised the selection cassette and generated this allele. (J:241137)
• Inheritance: Dominant

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdh23 Mutation: 281 strains or lines available

References

• Original: J:241137 Johnson KR, et al., Effects of Cdh23 single nucleotide substitutions on age-related hearing loss in C57BL/6 and 129S1/Sv mice and comparisons with congenic strains. Sci Rep. 2017 Mar 13;7:44450
• All: 5 reference(s)