Del(XMagea1-Magea6)1Nju
Targeted Allele Detail

Summary

• Symbol: Del(XMagea1-Magea6)1Nju
• Name: deletion, Chr X, Xiang Gao 1; deletion, Chr X, Model Animal Research Center for Nanjing University
• MGI ID: MGI:5898330
• Gene: Del(XMagea1-Magea6)1Nju Location: unknown Genetic Position: ChrX, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240206
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Del(XMagea1-Magea6)1Nju involves 6 genes/genome features (Magea6, Magea3, Magea8 ...) View all
• Mutation details: First, a loxP site flanked neomycin resistance gene cassette was inserted downstream of Magea1 via targeted recombination in ES cells (Igs18). The neo cassette was removed through subsequent cre-mediated recombination. Next, using ES cells targeted in the first stage, a loxP site and an FRT site flanked neo cassette were inserted upstream of Magea6 via targeted recombination (Igs19). The now floxed Mageac1 cluster was deleted through subsequent cre-mediated recombination. This knocks out all six Magea genes in this cluster: Magea6, Magea3, Magea8, Magea2, Magea5, and Magea1 and also removes the second neo cassette. RT-PCR confirmed the knockout of all six genes. (J:240206)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(XMagea1-Magea6)1Nju Mutation: 0 strains or lines available

References

• Original: J:240206 Hou S, et al., The Magea gene cluster regulates male germ cell apoptosis without affecting the fertility in mice. Sci Rep. 2016 May 26;6:26735
• All: 1 reference(s)