Gcg^{tm1.1(icre)Gkg}
Targeted Allele Detail

Summary

• Symbol: Gcg^{tm1.1(icre)Gkg}
• Name: glucagon; targeted mutation 1.1, George K Gittes
• MGI ID: MGI:5898434
• Synonyms: Gcg^iCre
• Gene: Gcg Location: Chr2:62304874-62313994 bp, - strand Genetic Position: Chr2, 35.85 cM
• Alliance: Gcg^{tm1.1(icre)Gkg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256199
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Gcg^{tm1.1(icre)Gkg} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gcg (MGI:95674)

• Mutation details: The targeting construct is designed to insert a codon improved Cre recombinase gene, followed by an FRT-flanked neomycin resistance (neo) cassette, into exon 2 of the gene. Unexpectedly the targeting process resulted in a duplication of all exons of Gcg gene, which spans 9 kb, and extends upstream for at least 17 kb and downstream for at least 7.8 kb, including the neighboring fibroblast activation protein (Fap) gene. Flp-mediated recombination removed the FRT-flanked neo cassette. Mice express Cre in 93-95% of alpha cells and in a negligible amount of beta cells. Cre expression is also seen in intestinal L cells and some neurons in brain. (J:256199)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gcg (mouse) Summary of all recombinase alleles driven by Gcg. Alliance mouse Gcg gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gcg Mutation: 34 strains or lines available

References

• Original: J:256199 Shiota C, et al., Gcg (CreERT2) knockin mice as a tool for genetic manipulation in pancreatic alpha cells. Diabetologia. 2017 Dec;60(12):2399-2408
• All: 12 reference(s)