Braf^tm1Tumg
Targeted Allele Detail

Summary

• Symbol: Braf^tm1Tumg
• Name: Braf transforming gene; targeted mutation 1, Yoko Aoki
• MGI ID: MGI:5902220
• Synonyms: Braf^{Q241R Neo}
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm1Tumg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216228
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A PGK-Neo-STOP cassette flanked by loxP sites was inserted in intron 6 and a glutamine to arginine substitution at amino acid 241 (Q241R) was inserted into exon 7 via homologous recombination. The mutant transcript is expressed only after cre-mediated recombination. (J:216228)

Disease models

Expression

In Structures Affected by this Mutation:

21 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 60 strains or lines available

References

• Original: J:216228 Inoue S, et al., New BRAF knockin mice provide a pathogenetic mechanism of developmental defects and a therapeutic approach in cardio-facio-cutaneous syndrome. Hum Mol Genet. 2014 Dec 15;23(24):6553-66
• All: 5 reference(s)