Slc12a3^{em1(cre/ERT2)Amc}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Slc12a3^{em1(cre/ERT2)Amc}
• Name: solute carrier family 12, member 3; endonuclease-mediated mutation 1, Andrew P McMahon
• MGI ID: MGI:5902998
• Synonyms: Slc12a3-IRES-CRE-ERT2
• Gene: Slc12a3 Location: Chr8:95055829-95092842 bp, + strand Genetic Position: Chr8, 46.46 cM
• Alliance: Slc12a3^{em1(cre/ERT2)Amc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159099
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Slc12a3^{em1(cre/ERT2)Amc} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc12a3 (MGI:108114)

• Mutation details: Plasmids encoding a signal guide RNA designed to introduce a cre/ERT2 knock-in mutation into 3' UTR near the stop codon of the Slc12a3 gene and the cas9 nuclease were introduced into the cytoplasm of C57BL/6N-derived JM8.N4 ES cells. (J:159099, J:354771)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc12a3 (mouse) Summary of all recombinase alleles driven by Slc12a3. Alliance mouse Slc12a3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc12a3 Mutation: 54 strains or lines available

References

• Original: J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010
• All: 2 reference(s)