Fgfr2tm2.1Sor
Targeted Allele Detail
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Symbol: |
Fgfr2tm2.1Sor |
Name: |
fibroblast growth factor receptor 2; targeted mutation 2.1, Philippe Soriano |
MGI ID: |
MGI:5903019 |
Synonyms: |
Fgfr2-mCherry, Fgfr2-T2A-H2B-mCherry |
Gene: |
Fgfr2 Location: Chr7:129764181-129868538 bp, - strand Genetic Position: Chr7, 73.19 cM
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Alliance: |
Fgfr2tm2.1Sor page
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Germline Transmission: |
Earliest citation of germline transmission:
J:241560
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Parent Cell Line: |
AK7.1 (ES Cell)
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Strain of Origin: |
129S4/SvJaeSor
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Allele Type: |
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Targeted (No functional change, Reporter) |
Mutation: |
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Insertion
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Mutation details: The targeting vector is designed to replace the stop codon in exon 19 of the Fgfr2 gene with a cassette containing a human HIST1H2BB gene [histone 1 H2bb] sequence, a viral 2A oligopeptide (T2A) that mediates ribosomal skipping, a red fluorescent protein (mCherry) sequence, and a new stop codon. An FRT-flanked neomycin resistance (neo) cassette was inserted in the intron upstream of exon 19. Flp-mediated recombination removed the FRT-flanked neo cassette.
(J:241560)
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Original: |
J:241560 Molotkov A, et al., Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency. Dev Cell. 2017 Jun 05;41(5):511-526.e4 |
All: |
9 reference(s) |
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