Dglucy^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dglucy^{tm1b(KOMP)Wtsi}
• Name: D-glutamate cyclase; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5903047
• Gene: Dglucy Location: Chr12:100745316-100838869 bp, + strand Genetic Position: Chr12, 51.1 cM
• Alliance: Dglucy^{tm1b(KOMP)Wtsi} page
• IMPC: Dglucy gene page

Mutation origin

• Mutant Cell Line: EPD0098_6_D10
• Germline Transmission: Earliest citation of germline transmission: J:241368
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 100804245 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 100805190. The critical exon(s) is/are thus flanked by loxP sites. This reporter knockout allele was created through cre-mediated recombination. Western blot analysis confirmed the absence of protein expression in the heart and other tissues. (J:241368)

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dglucy Mutation: 31 strains or lines available

References

• Original: J:241368 Ariyoshi M, et al., D-Glutamate is metabolized in the heart mitochondria. Sci Rep. 2017 Mar 07;7:43911
• All: 1 reference(s)