Gt(ROSA)26Sor^{tm1.1(CAG-Pycard/mCitrine*,-CD2*)Dtg}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-Pycard/mCitrine*,-CD2*)Dtg}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Douglas T Golenbock
• MGI ID: MGI:5903223
• Synonyms: ASC-citrine
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-Pycard/mCitrine*,-CD2*)Dtg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238511
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a loxP-flanked STOP cassette, the mASC-citrine-HA fusion protein (described below), an FRT site, an iIRES, a portion from the human CD2 antigen locus encoding a truncated signaling-deficient protein (hCD2*), a second FRT site and a BGH polyA signal. The targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The mASC-citrine-HA fusion protein cDNA sequence encodes the mouse PYD and CARD domain containing coding region fused to a Citrine variant of yellow fluorescent protein, all followed by a hemagglutinin tag sequence. The Citrine used here is a yellow fluorescent protein variant with the K69A and A206K mutations (for improved brightness and monomeric stability) - encoding a ~5.7 pKa monomer with ~488nm excitation and ~529nm emmission spectra. Cre-mediated recombination removed the upstream loxP-flanked STOP cassette. (J:238511)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:238511 Tzeng TC, et al., A Fluorescent Reporter Mouse for Inflammasome Assembly Demonstrates an Important Role for Cell-Bound and Free ASC Specks during In Vivo Infection. Cell Rep. 2016 Jul 12;16(2):571-82
• All: 18 reference(s)