Mpc1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mpc1^{tm1c(EUCOMM)Wtsi}
• Name: mitochondrial pyruvate carrier 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5903307
• Synonyms: Mcp1^fl
• Gene: Mpc1 Location: Chr17:8502590-8516499 bp, + strand Genetic Position: Chr17, 4.92 cM
• Alliance: Mpc1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mpc1 gene page

Mutation origin

• Mutant Cell Line: EPD0377_6_F04
• Germline Transmission: Earliest citation of germline transmission: J:236662
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 8514918 of Chromosome 17 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5 at position 8516753. The critical exons 3 through 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:236662)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mpc1 Mutation: 16 strains or lines available

References

• Original: J:236662 Gray LR, et al., Hepatic Mitochondrial Pyruvate Carrier 1 Is Required for Efficient Regulation of Gluconeogenesis and Whole-Body Glucose Homeostasis. Cell Metab. 2015 Oct 6;22(4):669-81
• All: 17 reference(s)