Npy^{tm1.1(flpo)Hze}
Targeted Allele Detail

Summary

• Symbol: Npy^{tm1.1(flpo)Hze}
• Name: neuropeptide Y; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5903980
• Synonyms: Npy-IRES2-FlpO
• Gene: Npy Location: Chr6:49799690-49806487 bp, + strand Genetic Position: Chr6, 24.04 cM, cytoband B3
• Alliance: Npy^{tm1.1(flpo)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260362
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Npy^{tm1.1(flpo)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Npy (MGI:97374)

• Mutation details: The targeting vector contains, from 5' to 3', a partial Npy sequence spanning exon 4 (including the endogenous stop codon), an internal ribosome entry site 2 sequence, a FlpO recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). (J:260362)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Npy (mouse) Summary of all recombinase alleles driven by Npy. Alliance mouse Npy gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Npy Mutation: 19 strains or lines available

References

• Original: J:260362 Daigle TL, et al., A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell. 2018 Jul 12;174(2):465-480.e22
• All: 17 reference(s)