Slc17a6^{tm1.1(flpo)Hze}
Targeted Allele Detail

Summary

• Symbol: Slc17a6^{tm1.1(flpo)Hze}
• Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5903982
• Synonyms: Slc17a6-IRES2-FlpO-D, Vglut2-IRES2-FLPo-D
• Gene: Slc17a6 Location: Chr7:51271754-51320867 bp, + strand Genetic Position: Chr7, 32.78 cM, cytoband B4
• Alliance: Slc17a6^{tm1.1(flpo)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Slc17a6^{tm1.1(flpo)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc17a6 (MGI:2156052)

• Mutation details: The targeting vector contains, from 5' to 3', a partial Slc17a6 sequence spanning exon 12 (including the endogenous stop codon), an internal ribosome entry site 2 sequence, a FlpO recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc17a6 (mouse) Summary of all recombinase alleles driven by Slc17a6. Alliance mouse Slc17a6 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc17a6 Mutation: 60 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 12 reference(s)