Esr2^{tm1.1(cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Esr2^{tm1.1(cre)Hze}
• Name: estrogen receptor 2 (beta); targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5904044
• Synonyms: Esr2-IRES2-Cre-D
• Gene: Esr2 Location: Chr12:76167193-76224033 bp, - strand Genetic Position: Chr12, 33.52 cM, cytoband D1-D3
• Alliance: Esr2^{tm1.1(cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146821
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Esr2^{tm1.1(cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Esr2 (MGI:109392)

• Mutation details: The targeting vector contained, from 5' to 3', a partial Esr2 sequence spanning exon 10 (including the endogenous stop codon), an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::FRT5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). The resulting Esr2-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice. Cre recombinase expression/activity largely recapitulates the endogenous Esr2 gene expression in the brain - observed mainly in medial amygdalar nucleus, bed nuclei of the stria terminalis, paraventricular hypothalamic nucleus, cortical layer 6 and scattered neurons elsewhere in the brain. (J:146821)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Esr2 (mouse) Summary of all recombinase alleles driven by Esr2. Alliance mouse Esr2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Esr2 Mutation: 36 strains or lines available

References

• Original: J:146821 Allen Institute for Brain Science, Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS). MGI Direct Data Submission. 2009-04-06
• All: 10 reference(s)