Gt(ROSA)26Sor^{tm11(CAG-tdTomato*,-GFP*)Nat}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm11(CAG-tdTomato*,-GFP*)Nat}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 11, Jeremy Nathans
• MGI ID: MGI:5904987
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm11(CAG-tdTomato*,-GFP*)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269489
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: This knock-in line at the Gt(ROSA26)Sor locus carries the following genetic elements (listed in order from 5' to 3'): CAG promoter, loxP-3x polyadenylation site-loxP ("lox-stop-lox"), membrane-anchored (via an N-terminal myristoylation site) modified tdTomato with a C-terminal triple-myc tag, 2A cleavage site, histone H2B-GFP fusion with C-terminal triple hemagglutinin tag, bovine growth hormone gene 3'UTR, and Frt-PGK-Neo-Frt. In the absence of Cre-mediated recombination, neither the tdTomato nor the GFP reporter is expressed. Upon crossing to a Cre recombinase-expressing transgene or knock-in, the lox-stop-lox cassette is excised in cells that express Cre, leading to production of membrane-associated tdTomato and nuclear H2B-GFP. The fluorescent proteins can be visualized based on their intrinsic fluorescence, or by immunostaining for the fluorescent proteins and/or their associated epitope tags. (J:269489)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:269489 Wang Y, et al., Interplay of the Norrin and Wnt7a/Wnt7b signaling systems in blood-brain barrier and blood-retina barrier development and maintenance. Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11827-E11836
• All: 3 reference(s)