Ampd1^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ampd1^{tm1d(KOMP)Wtsi}
• Name: adenosine monophosphate deaminase 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5905189
• Synonyms: Ampd1^tm1d
• Gene: Ampd1 Location: Chr3:102981330-103007036 bp, + strand Genetic Position: Chr3, 45.25 cM
• Alliance: Ampd1^{tm1d(KOMP)Wtsi} page
• IMPC: Ampd1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240489
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 102985923 of Chromosome 3 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 102986789. Exon is thus flanked by loxP sites. A "conditional ready" (floxed) allele was then created by flp recombinase expression in mice carrying this allele, which removes the lacZ and neo cassettes. The knockout allele was created by deleting exon 3 through subsequent cre-mediated recombination. (J:204739, J:240489)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ampd1 Mutation: 49 strains or lines available

References

• Original: J:240489 Pan Y, et al., Insertion of a knockout-first cassette in Ampd1 gene leads to neonatal death by disruption of neighboring genes expression. Sci Rep. 2016 Oct 24;6:35970
• All: 2 reference(s)