Cfap69^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cfap69^{tm1c(KOMP)Wtsi}
• Name: cilia and flagella associated protein 69; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5906136
• Synonyms: Cfap69^flox
• Gene: Cfap69 Location: Chr5:5629284-5714232 bp, - strand Genetic Position: Chr5, 3.07 cM
• Alliance: Cfap69^{tm1c(KOMP)Wtsi} page
• IMPC: Cfap69 gene page

Mutation origin

• Mutant Cell Line: EPD0713_1_E05
• Germline Transmission: Earliest citation of germline transmission: J:242686
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 5694180 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 5694950. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:242686)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cfap69 Mutation: 58 strains or lines available

References

• Original: J:242686 Talaga AK, et al., Cilia- and Flagella-Associated Protein 69 Regulates Olfactory Transduction Kinetics in Mice. J Neurosci. 2017 Jun 07;37(23):5699-5710
• All: 1 reference(s)