Rhot1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rhot1^{tm1c(EUCOMM)Wtsi}
• Name: ras homolog family member T1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5906207
• Gene: Rhot1 Location: Chr11:80099845-80158733 bp, + strand Genetic Position: Chr11, 47.62 cM, cytoband B5
• Alliance: Rhot1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Rhot1 gene page

Mutation origin

• Mutant Cell Line: EPD0066_2_F01
• Germline Transmission: Earliest citation of germline transmission: J:240500
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 80110547 of Chromosome 11 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 2 at position 80111572. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:240500)

Expression

In Mice Carrying this Mutation:	102 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rhot1 Mutation: 44 strains or lines available

References

• Original: J:240500 Lopez-Domenech G, et al., Loss of Dendritic Complexity Precedes Neurodegeneration in a Mouse Model with Disrupted Mitochondrial Distribution in Mature Dendrites. Cell Rep. 2016 Oct 04;17(2):317-327
• All: 2 reference(s)