Il22^{tm1.1(cre)Lky}
Targeted Allele Detail

Summary

• Symbol: Il22^{tm1.1(cre)Lky}
• Name: interleukin 22; targeted mutation 1.1, Richard M Locksley
• MGI ID: MGI:5906262
• Gene: Il22 Location: Chr10:118040847-118045952 bp, + strand Genetic Position: Chr10, 66.67 cM
• Alliance: Il22^{tm1.1(cre)Lky} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242759
• Parent Cell Line: PRX-B6T (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutation: Insertion
• Il22^{tm1.1(cre)Lky} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Il22 (MGI:1355307)

• Mutation details: Rabbit beta-globin (partial exon 2-3 genomic sequence serving as an artificial intron and splicing donor/acceptor), tandem dimer red fluorescent protein (tdTomato), encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES), huCre (P1 phage cre-recombinase cDNA optimized for human/mammalian cells), bovine growth hormone (bGH) poly-adenylation signal (pA), and a loxP-flanked neomycin cassette were introduced to exon 1 of the mouse Il22 gene via homologous recombination in embryonic stem (ES) cells. The floxed neomycin cassette was excised by through crosses with CMV-cre mice. Mice express tdTomato fluorescent reporter and cre in cells where Il22 is expressed. (J:242759)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Il22 (mouse) Summary of all recombinase alleles driven by Il22. Alliance mouse Il22 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il22 Mutation: 27 strains or lines available

References

• Original: J:242759 Savage AK, et al., The Development of Steady-State Activation Hubs between Adult LTi ILC3s and Primed Macrophages in Small Intestine. J Immunol. 2017 Sep 1;199(5):1912-1922
• All: 1 reference(s)