Nsmce2^tm1.1Ofc
Targeted Allele Detail

Summary

• Symbol: Nsmce2^tm1.1Ofc
• Name: NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase; targeted mutation 1.1, Oscar Fernandez-Capetillo
• MGI ID: MGI:5906663
• Synonyms: Nsmce2^SD
• Gene: Nsmce2 Location: Chr15:59246096-59473533 bp, + strand Genetic Position: Chr15, 25.19 cM
• Alliance: Nsmce2^tm1.1Ofc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227197
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The SUMO ligase activity of the gene was inactivated by introduction of mutations encoding C195S and H197A in intron 6 and an FRT flanked pgk-neo cassette was inserted via homologous recombination. Flp mediated recombination removed the neo cassette. In vitro auto-SUMOylation assays confirmed the absence of SUMO ligase activity. However, protein levels and the stability of SMC5/6 complexes are similar to wild-type protein. (J:227197)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nsmce2 Mutation: 48 strains or lines available

References

• Original: J:227197 Jacome A, et al., NSMCE2 suppresses cancer and aging in mice independently of its SUMO ligase activity. EMBO J. 2015 Nov 3;34(21):2604-19
• All: 1 reference(s)