Abhd6^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Abhd6^{tm1c(EUCOMM)Hmgu}
• Name: abhydrolase domain containing 6; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5907201
• Synonyms: ABHD6^flox
• Gene: Abhd6 Location: Chr14:14413010-14466871 bp, - strand Genetic Position: Chr14, 4.69 cM
• Alliance: Abhd6^{tm1c(EUCOMM)Hmgu} page
• IMPC: Abhd6 gene page

Mutation origin

• Mutant Cell Line: HEPD0651_8_C07
• Germline Transmission: Earliest citation of germline transmission: J:215257
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 14426699 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 14429075. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:215257)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Abhd6 Mutation: 35 strains or lines available

References

• Original: J:215257 Zhao S, et al., alpha/beta-Hydrolase domain-6-accessible monoacylglycerol controls glucose-stimulated insulin secretion. Cell Metab. 2014 Jun 3;19(6):993-1007
• All: 4 reference(s)