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Cfap157tm1d(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Cfap157tm1d(EUCOMM)Hmgu
Name: cilia and flagella associated protein 157; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:5907283
Synonyms: Cfap157deltaex2
Gene: Cfap157  Location: Chr2:32667425-32674417 bp, - strand  Genetic Position: Chr2, 22.09 cM
Alliance: Cfap157tm1d(EUCOMM)Hmgu page
IMPC: Cfap157 gene page
Mutation
origin
Mutant Cell Line:  HEPD0601_7_G05
Germline Transmission:  Earliest citation of germline transmission: J:237484
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 32671182 of Chromosome 2 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 32671954. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 2. RT-PCR, northern blot and immunoprecipitation experiments confirmed the lack of wild-type expression from this allele. qRT-PCR did detect transcripts lacking exon 2 or exon 2 and 3, but no peptides were detected. (J:237484)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cfap157 Mutation:  31 strains or lines available
References
Original:  J:237484 Weidemann M, et al., CFAP157 is a murine downstream effector of FOXJ1 that is specifically required for flagellum morphogenesis and sperm motility. Development. 2016 Dec 15;143(24):4736-4748
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory