Cfap157tm1d(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Cfap157tm1d(EUCOMM)Hmgu |
Name: |
cilia and flagella associated protein 157; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5907283 |
Synonyms: |
Cfap157deltaex2 |
Gene: |
Cfap157 Location: Chr2:32667425-32674417 bp, - strand Genetic Position: Chr2, 22.09 cM
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Alliance: |
Cfap157tm1d(EUCOMM)Hmgu page
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IMPC: |
Cfap157 gene page |
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Mutant Cell Line: |
HEPD0601_7_G05 |
Germline Transmission: |
Earliest citation of germline transmission:
J:237484
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 32671182 of Chromosome 2 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 32671954. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 2. RT-PCR, northern blot and immunoprecipitation experiments confirmed the lack of wild-type expression from this allele. qRT-PCR did detect transcripts lacking exon 2 or exon 2 and 3, but no peptides were detected.
(J:237484)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:237484 Weidemann M, et al., CFAP157 is a murine downstream effector of FOXJ1 that is specifically required for flagellum morphogenesis and sperm motility. Development. 2016 Dec 15;143(24):4736-4748 |
All: |
1 reference(s) |
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