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Zscan4ctm1Kom
Targeted Allele Detail
Summary
Symbol: Zscan4ctm1Kom
Name: zinc finger and SCAN domain containing 4C; targeted mutation 1, Minoru Ko
MGI ID: MGI:5907620
Synonyms: Z4c-Emerald-KI, Zscan4c-Emerald-Neo+
Gene: Zscan4c  Location: Chr7:10739672-10744474 bp, + strand  Genetic Position: Chr7, 6.77 cM
Alliance: Zscan4ctm1Kom page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:241396
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  (C57BL/6J x 129S6/SvEvTac)F1
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsMost of exon 2 (from the ATG start codon onwards) and intron 2 was replaced with the emerald GFP gene and polyA site, a loxP site and an FRT site flanked neomycin resistance gene cassette. This creates a reporter and null allele with the target gene knocked-out and the GFP under the control of its promoter. Because of the high sequence similarity between the paralogs in the gene cluster where the target gene is located, the targeted mutation strategy was combined with an endonuclease-mediated mutation strategy. The targeting vector was transfected into ES cells together with a CRISPR/CAS9 vector encoding two sgRNAs directed to the proximal half of intron 2. (J:241396)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Zscan4c Mutation:  10 strains or lines available
Notes
ES cell line = TA1.
References
Original:  J:241396 Ishiguro KI, et al., Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos. In Vitro Cell Dev Biol Anim. 2017 Feb;53(2):179-190
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory