Zscan4ctm1Kom
Targeted Allele Detail
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Symbol: |
Zscan4ctm1Kom |
Name: |
zinc finger and SCAN domain containing 4C; targeted mutation 1, Minoru Ko |
MGI ID: |
MGI:5907620 |
Synonyms: |
Z4c-Emerald-KI, Zscan4c-Emerald-Neo+ |
Gene: |
Zscan4c Location: Chr7:10739672-10744474 bp, + strand Genetic Position: Chr7, 6.77 cM
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Alliance: |
Zscan4ctm1Kom page
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Most of exon 2 (from the ATG start codon onwards) and intron 2 was replaced with the emerald GFP gene and polyA site, a loxP site and an FRT site flanked neomycin resistance gene cassette. This creates a reporter and null allele with the target gene knocked-out and the GFP under the control of its promoter. Because of the high sequence similarity between the paralogs in the gene cluster where the target gene is located, the targeted mutation strategy was combined with an endonuclease-mediated mutation strategy. The targeting vector was transfected into ES cells together with a CRISPR/CAS9 vector encoding two sgRNAs directed to the proximal half of intron 2.
(J:241396)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Zscan4c Mutation: |
10 strains or lines available
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Original: |
J:241396 Ishiguro KI, et al., Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos. In Vitro Cell Dev Biol Anim. 2017 Feb;53(2):179-190 |
All: |
1 reference(s) |
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