Zscan4c^tm1.1Kom
Targeted Allele Detail

Summary

• Symbol: Zscan4c^tm1.1Kom
• Name: zinc finger and SCAN domain containing 4C; targeted mutation 1.1, Minoru Ko
• MGI ID: MGI:5907623
• Synonyms: Zscan4c-Emerald-NeoDelta
• Gene: Zscan4c Location: Chr7:10739672-10744474 bp, + strand Genetic Position: Chr7, 6.77 cM
• Alliance: Zscan4c^tm1.1Kom page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241396
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exon 2 (from the ATG start codon onwards) and intron 2 was replaced with the emerald GFP gene and polyA site, a loxP site and an FRT site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent flp-mediated recombination. This creates a reporter and null allele with the target gene knocked-out and the GFP under the control of its promoter. Because of the high sequence similarity between the parologs in the gene cluster where the target gene is located, the targeted mutation strategy was combined with an endonuclease-mediated mutation strategy. The targeting vector was transfected into ES cells together with a CRISPR/CAS9 vector encoding two sgRNAs directed to the proximal half of intron 2. (J:241396)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zscan4c Mutation: 10 strains or lines available

Notes

ES cell line = TA1.

References

• Original: J:241396 Ishiguro KI, et al., Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos. In Vitro Cell Dev Biol Anim. 2017 Feb;53(2):179-190
• All: 1 reference(s)