Uhrf2em1Yoz
Endonuclease-mediated Allele Detail
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| Symbol: |
Uhrf2em1Yoz |
| Name: |
ubiquitin-like, containing PHD and RING finger domains 2; endonuclease-mediated mutation 1, Yongxiang Zhao |
| MGI ID: |
MGI:5907901 |
| Gene: |
Uhrf2 Location: Chr19:30007920-30071126 bp, + strand Genetic Position: Chr19, 24.73 cM
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| Alliance: |
Uhrf2em1Yoz page
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| Allele Type: |
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Endonuclease-mediated (Null/knockout) |
| Mutation: |
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Intragenic deletion
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Mutation details: Two sgRNAs (5'-GGTCCGCACCATCGACGGCTCC-3' and 5'-GGAGCTGCGCGAGCGGGTCT-3') were used to target the start of the coding region of exon 1 with CRISPR/Cas9. A number of different deletions were found, with this allele, number 10, representing a 277 bp deletion removing part of the 5' UTR and the start of the coding region (-180 to +97). The absence of transcripts from this allele was confirmed by qPCR and the absence of peptides by Western blot analysis of thymus and brain samples.
(J:241384, J:243187)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Uhrf2 Mutation: |
36 strains or lines available
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| Original: |
J:243187 Li D, et al., Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol. 2013 Aug;31(8):681-3 |
| All: |
3 reference(s) |
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Analysis Tools
