Krt14^{tm1.1(cre/ERT2)Hjg}
Targeted Allele Detail

Summary

• Symbol: Krt14^{tm1.1(cre/ERT2)Hjg}
• Name: keratin 14; targeted mutation 1.1, Hermann-Josef Grone
• MGI ID: MGI:5908393
• Synonyms: K14CreERT2, Krt14^{tm1.1(cre/ERT2)Dkfz}
• Gene: Krt14 Location: Chr11:100093988-100098336 bp, - strand Genetic Position: Chr11, 63.43 cM, cytoband D
• Alliance: Krt14^{tm1.1(cre/ERT2)Hjg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201085
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Krt14^{tm1.1(cre/ERT2)Hjg} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Krt14 (MGI:96688)

• Mutation details: An icre/ERT2 cassette followed by an Frt-flanked neo selection cassette was inserted into downstream of ATG-start codon in frame by homologous recombination in ES cells. Correctly targeted ES cell clones were injected into C57BL/6 blastocyts. Mice were crossed to Flp-expressing mice to delete the neo cassette. (J:201085)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Krt14 (mouse) Summary of all recombinase alleles driven by Krt14. Comparative matrix view of recombinase activities Alliance mouse Krt14 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Krt14 Mutation: 38 strains or lines available

References

• Original: J:201085 Amen N, et al., Differentiation of epidermal keratinocytes is dependent on glucosylceramide:ceramide processing. Hum Mol Genet. 2013 Oct 15;22(20):4164-79
• All: 1 reference(s)