Pcbp2^tm1.2Salr
Targeted Allele Detail

Summary

• Symbol: Pcbp2^tm1.2Salr
• Name: poly(rC) binding protein 2; targeted mutation 1.2, Stephen A Liebhaber
• MGI ID: MGI:5908914
• Gene: Pcbp2 Location: Chr15:102378974-102408496 bp, + strand Genetic Position: Chr15, 57.61 cM, cytoband F1
• Alliance: Pcbp2^tm1.2Salr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235912
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of the transcription initiation site. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 1 and 2. Western blot analysis confirmed the absence of protein expression in embryos. (J:235912)

Expression

In Mice Carrying this Mutation:	52018 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcbp2 Mutation: 60 strains or lines available

References

• Original: J:235912 Ghanem LR, et al., The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development. Mol Cell Biol. 2015 Nov 02;36(2):304-19
• All: 3 reference(s)