Stag2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Stag2^{tm1c(EUCOMM)Wtsi}
• Name: STAG2 cohesin complex component; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5909046
• Synonyms: Stag2^flox
• Gene: Stag2 Location: ChrX:41238194-41366062 bp, + strand Genetic Position: ChrX, 23.19 cM, cytoband A2
• Alliance: Stag2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Stag2 gene page

Mutation origin

• Mutant Cell Line: EPD0057_4_D12
• Germline Transmission: Earliest citation of germline transmission: J:278649
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted upstream of the critical exon (exon 7) with insertion at bp position ChrX:42,222,777 [Build GRCm38]. The L1L2_gt0 cassette is composed of an FRT site, a mouse En2 intron/splice-acceptor/exon (En2 SA), a self-cleaving 2A peptide, the beta-galactosidase gene (lacZ), a second self-cleaving 2A peptide, the neomycin-resistance gene (neo), an SV40 polyadenylation signal (SV40pA), a second FRT site and a loxP site. A second loxP site was inserted downstream of exon 7 at bp position ChrX:42,223,727 [Build GRCm38]; therefore flanking exon 7 with loxP sites. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 7 floxed. (J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Stag2 Mutation: 15 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 6 reference(s)