Stag1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Stag1^{tm1c(EUCOMM)Hmgu}
• Name: STAG1 cohesin complex component; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5909049
• Synonyms: Stag1^flox
• Gene: Stag1 Location: Chr9:100479762-100840597 bp, + strand Genetic Position: Chr9, 52.77 cM, cytoband F1
• Alliance: Stag1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Stag1 gene page

Mutation origin

• Mutant Cell Line: HEPD0684_4_H05
• Germline Transmission: Earliest citation of germline transmission: J:278649
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted upstream of the critical exon (exon 7) of the stromal antigen 1 locus (Stag1) - with insertion at bp position Chr9:100,785,969 [Build GRCm38]. The L1L2_Bact_P cassette was composed of an FRT site, a mouse En2 intron/splice-acceptor/exon (En2 SA), an IRES sequence, the beta-galactosidase gene (lacZ) with SV40 polyadenylation signal (SV40pA), a loxP site, the human beta actin promoter-driven neomycin-resistance gene with SV40 polyadenylation signal (hbactP-neo-pA), a second FRT site and a second loxP site. A third loxP site was inserted downstream of exon 7 at bp position Chr9:100,786,980 [Build GRCm38]; therefore flanking exon7 with loxP sites. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 7 floxed. (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Stag1 Mutation: 105 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)