Slc8b1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc8b1^{tm1c(EUCOMM)Wtsi}
• Name: solute carrier family 8 (sodium/lithium/calcium exchanger), member B1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5909410
• Synonyms: Slc8b1^fl
• Gene: Slc8b1 Location: Chr5:120649233-120672089 bp, + strand Genetic Position: Chr5, 60.63 cM
• Alliance: Slc8b1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Slc8b1 gene page

Mutation origin

• Mutant Cell Line: EPD0460_4_A08
• Germline Transmission: Earliest citation of germline transmission: J:241501
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 120658187 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 120659420. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:241501)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc8b1 Mutation: 47 strains or lines available

References

• Original: J:241501 Luongo TS, et al., The mitochondrial Na+/Ca2+ exchanger is essential for Ca2+ homeostasis and viability. Nature. 2017 May 04;545(7652):93-97
• All: 6 reference(s)