Tmigd3^{Tg(H2-K-CALR*)del52Shmd}
Transgenic Allele Detail

Summary

• Symbol: Tmigd3^{Tg(H2-K-CALR*)del52Shmd}
• Name: transmembrane and immunoglobulin domain containing 3; transgene insertion del52, Kazuya Shimoda
• MGI ID: MGI:5909983
• Synonyms: CALRdel52, Tg(H2-K-CALR*)del52Shmd
• Gene: Tmigd3 Location: Chr3:105778219-105831358 bp, + strand Genetic Position: Chr3, Syntenic
• Alliance: Tmigd3^{Tg(H2-K-CALR*)del52Shmd} page

Transgene origin

• Strain of Origin: B6D2F1

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Tmigd3^{Tg(H2-K-CALR*)del52Shmd} expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CALR (811)

  Calr	(MGI:88252)

  type 1 mutation (del52) with 52 bp deletion found in essential thrombocythemia (ET) patients

• Mutation details: The transgene is constructed with a mouse promoter and a mutated human cDNA, using the pSP65-H2K-i-LTR vector. The human cDNA is for the type 1 mutation (del52) that has a 52 bp deletion in exon 9, commonly found in essential thrombocythemia (ET) patients. Two copies of the transgene were found to have inserted head-to-head into intron 5 of the host gene. RT-PCR experiments confirmed expression from the transgene. (J:241724)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmigd3 Mutation: 6 strains or lines available

References

• Original: J:241724 Shide K, et al., Calreticulin mutant mice develop essential thrombocythemia that is ameliorated by the JAK inhibitor ruxolitinib. Leukemia. 2017 May;31(5):1136-1144
• All: 2 reference(s)