Nlrp9b^em1Flv
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nlrp9b^em1Flv
• Name: NLR family, pyrin domain containing 9B; endonuclease-mediated mutation 1, Richard Flavell
• MGI ID: MGI:5910440
• Gene: Nlrp9b Location: Chr7:19725318-19796867 bp, + strand Genetic Position: Chr7, 9.96 cM
• Alliance: Nlrp9b^em1Flv page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 2 and part of exon 3 were deleted using sgRNAs and the CRISPR/Cas9 system. This region contains the common translation initiation codon for two different isoforms of the encoded protein. qRT-PCR validation using intestine tissues showed that no detectable full length transcript is expressed from this allele. (J:243487)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nlrp9b Mutation: 51 strains or lines available

References

• Original: J:243487 Zhu S, et al., Nlrp9b inflammasome restricts rotavirus infection in intestinal epithelial cells. Nature. 2017 Jun 29;546(7660):667-670
• All: 1 reference(s)