Washc5^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Washc5^{tm1c(EUCOMM)Hmgu}
• Name: WASH complex subunit 5; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5910638
• Synonyms: Strumpelin f/f
• Gene: Washc5 Location: Chr15:59203846-59246016 bp, - strand Genetic Position: Chr15, 25.17 cM
• Alliance: Washc5^{tm1c(EUCOMM)Hmgu} page
• IMPC: Washc5 gene page

Mutation origin

• Mutant Cell Line: HEPD0534_7_C01
• Germline Transmission: Earliest citation of germline transmission: J:240360
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 59223060 of Chromosome 15 upstream of the critical exon 12 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 59223919. The critical exon 12 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:240360)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Washc5 Mutation: 58 strains or lines available

References

• Original: J:240360 Tyrrell BJ, et al., Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell Melanoma Res. 2016 Sep;29(5):559-71
• All: 2 reference(s)