Gcna^tm1.1Dcp
Targeted Allele Detail

Summary

• Symbol: Gcna^tm1.1Dcp
• Name: germ cell nuclear acidic protein; targeted mutation 1.1, David C Page
• MGI ID: MGI:5910931
• Gene: Gcna Location: ChrX:100739442-100749805 bp, + strand Genetic Position: ChrX, Syntenic
• Alliance: Gcna^tm1.1Dcp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235710
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: An FRT-lacZ-loxP-Neomycin/pA-FRT-loxP cassette was placed in intron 3 and a loxP site was introduced to intron 4 via homologous recombination. Transient infection of the ES cells with a plasmid expressing FlpE (pCAGGS-FlpE-PURO) excised the lacZ and neomycin components, leaving exon 4 flanked by loxP sites. (J:235710)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gcna Mutation: 1 strain or line available

References

• Original: J:235710 Carmell MA, et al., A widely employed germ cell marker is an ancient disordered protein with reproductive functions in diverse eukaryotes. Elife. 2016 Oct 08;5:e19993
• All: 4 reference(s)