Fos^{tm2.1(icre/ERT2)Luo}
Targeted Allele Detail

Summary

• Symbol: Fos^{tm2.1(icre/ERT2)Luo}
• Name: FBJ osteosarcoma oncogene; targeted mutation 2.1, Liqun Luo
• MGI ID: MGI:5910950
• Synonyms: Fos^2A-iCreER, Fos^2A-iCreERT2, TRAP2
• Gene: Fos Location: Chr12:85520664-85524047 bp, + strand Genetic Position: Chr12, 39.7 cM
• Alliance: Fos^{tm2.1(icre/ERT2)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Fos^{tm2.1(icre/ERT2)Luo} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Fos (MGI:95574)

• Mutation details: A targeting vector was designed to insert a 2A::iCreER::frt-neo-frt cassette into the translational stop site of the FBJ osteosarcoma oncogene locus (Fos) on chromosome 12. The viral 2A oligopeptide sequence mediates ribosomal skipping. The iCreER fusion gene used here has an improved/optimized variant of Cre recombinase (iCre) that is fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain (ER). TThe targeting construct was electroporated into 129;FVB-derived embryonic stem (ES) cells, and correctly targeted ES cells were injected into recipient blastocysts. Chimeric animals were bred to CD1 mice and/or germline-active GFP-FlpO transgenic mice (mixed genetic background including CD1) to delete the frt-flanked neo cassette. (J:101977, J:250613)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Fos (mouse) Summary of all recombinase alleles driven by Fos. Comparative matrix view of recombinase activities Alliance mouse Fos gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fos Mutation: 43 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 73 reference(s)