Tg(Tff1-cre/ERT2,Tff2-rtTA,Tff3-luc)#Ern
Transgene Detail

Summary

• Symbol: Tg(Tff1-cre/ERT2,Tff2-rtTA,Tff3-luc)#Ern
• Name: transgene insertion, Matthias Ernst
• MGI ID: MGI:5911344
• Synonyms: Tg(Tff1-CreERT2;Tff2-rtTA;Tff3-Luc)
• Transgene: Tg(Tff1-cre/ERT2,Tff2-rtTA,Tff3-luc)#Ern Location: unknown
• Alliance: Tg(Tff1-cre/ERT2,Tff2-rtTA,Tff3-luc)#Ern page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Reporter, Transactivator)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Tff1-cre/ERT2,Tff2-rtTA,Tff3-luc)#Ern expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tff1 (MGI:88135)

• Mutation details: The transgene was generated by using a BAC of the clone RP23-371G13 which harbors the three Tff genes (Tff1, Tff2, Tff3) into which an inducible cre recombinase gene (cre/ERT2), the optimized reverse tetracycline/doxycycline-controlled transactivator (rtTA), and the coding sequence of the firefly luciferase (Luc) were introduced at the genomic sequence immediately upstream of the ATG start codon of the endogenous Tff1, Tff2, and Tff3 genes, respectively with each expression cassette followed by polyadenylation (pA)-signal. Four lines were generated with similar expression patterns. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:237622)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tff1 (mouse) Summary of all recombinase alleles driven by Tff1. Alliance mouse Tff1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:237622 Thiem S, et al., Inducible gene modification in the gastric epithelium of Tff1-CreERT2, Tff2-rtTA, Tff3-luc mice. Genesis. 2016 Dec;54(12):626-635
• All: 1 reference(s)