Rnaseh2b^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnaseh2b^{tm1c(EUCOMM)Wtsi}
• Name: ribonuclease H2, subunit B; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5911393
• Synonyms: Rnaseh2b^FLOX
• Gene: Rnaseh2b Location: Chr14:62569517-62610445 bp, + strand Genetic Position: Chr14, 33.19 cM, cytoband C3
• Alliance: Rnaseh2b^{tm1c(EUCOMM)Wtsi} page
• IMPC: Rnaseh2b gene page

Mutation origin

• Mutant Cell Line: EPD0087_4_A02
• Germline Transmission: Earliest citation of germline transmission: J:186986
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 62590506 of Chromosome 14 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 5 at position 62591370. The critical exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:186986)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnaseh2b Mutation: 43 strains or lines available

References

• Original: J:186986 Hiller B, et al., Mammalian RNase H2 removes ribonucleotides from DNA to maintain genome integrity. J Exp Med. 2012 Jul 30;209(8):1419-26
• All: 10 reference(s)