Mettl3^tm1Jhha
Targeted Allele Detail

Summary

• Symbol: Mettl3^tm1Jhha
• Name: methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit; targeted mutation 1, Jacob Hanna
• MGI ID: MGI:5911703
• Gene: Mettl3 Location: Chr14:52532298-52542585 bp, - strand Genetic Position: Chr14, 26.88 cM, cytoband C1
• Alliance: Mettl3^tm1Jhha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219587
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: KOMP vector PG00144_Z_5_D09 inserted at position 52299195 of Chromosome 14 upstream of the critical exon 4 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 52298473. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:219587)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mettl3 Mutation: 42 strains or lines available

References

• Original: J:219587 Geula S, et al., Stem cells. m6A mRNA methylation facilitates resolution of naive pluripotency toward differentiation. Science. 2015 Feb 27;347(6225):1002-6
• All: 7 reference(s)