Ythdf2^tm1.2Doca
Targeted Allele Detail

Summary

• Symbol: Ythdf2^tm1.2Doca
• Name: YTH N6-methyladenosine RNA binding protein 2; targeted mutation 1.2, Donal OCarroll
• MGI ID: MGI:5911734
• Gene: Ythdf2 Location: Chr4:131912227-131939567 bp, - strand Genetic Position: Chr4, 64.92 cM
• Alliance: Ythdf2^tm1.2Doca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243747
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing an EGFP reporter gene, PreScission protease cleavage site and several epitope tags (6 histidine, FLAG and 2 HA) was inserted into exon 1 at the ATG start site. Exon 2 was flanked by loxP sites. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted upstream of exon 2. Cre-mediated recombination removed exon 2. (J:243747)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ythdf2 Mutation: 32 strains or lines available

References

• Original: J:243747 Ivanova I, et al., The RNA m6A Reader YTHDF2 Is Essential for the Post-transcriptional Regulation of the Maternal Transcriptome and Oocyte Competence. Mol Cell. 2017 Sep 21;67(6):1059-1067.e4
• All: 1 reference(s)