Gt(ROSA)26Sor^{tm1(CAG-hVOS1.5)Mbja}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-hVOS1.5)Mbja}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Meyer Jackson
• MGI ID: MGI:5911835
• Synonyms: Ai35-hVOS, hVOS
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-hVOS1.5)Mbja} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243750
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), the hVOS1.5 DNA sequence (described below), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. The entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. hVOS1.5 is an optimized hybrid voltage sensor consisting of cerulean fluorescent protein (CeFP) with a C-terminal truncated h-ras farnesylation motif that tethers it to the inner face of the plasma membrane. (J:243750)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:243750 Bayguinov PO, et al., Imaging Voltage in Genetically Defined Neuronal Subpopulations with a Cre Recombinase-Targeted Hybrid Voltage Sensor. J Neurosci. 2017 Sep 20;37(38):9305-9319
• All: 3 reference(s)