Slc22a6^{tm1.1(cre/ERT2)Amat}
Targeted Allele Detail

Summary

• Symbol: Slc22a6^{tm1.1(cre/ERT2)Amat}
• Name: solute carrier family 22 (organic anion transporter), member 6; targeted mutation 1.1, Athena Matakidou
• MGI ID: MGI:5911847
• Synonyms: Slc22a6-CreER^T2
• Gene: Slc22a6 Location: Chr19:8595403-8605663 bp, + strand Genetic Position: Chr19, 5.44 cM
• Alliance: Slc22a6^{tm1.1(cre/ERT2)Amat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243751
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Slc22a6^{tm1.1(cre/ERT2)Amat} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc22a6 (MGI:892001)

• Mutation details: Using recombineering methods, the CreERT2-Frt-PGK-Hygromycin-pA-Frt cassette replaced the ATG start codon in exon 1 of the Slc22a6 gene in the BAC clone RP23-457i11. The modified BAC construct was electroporated into ES cells and correctly targeted ES cells were injected into CD1 blastocysts and the resulting chimeric mice were bred to C57BL/6J mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked hygromycin selection cassette. (J:243751)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc22a6 (mouse) Summary of all recombinase alleles driven by Slc22a6. Alliance mouse Slc22a6 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Slc22a6 Mutation: 34 strains or lines available

Notes

C57BL/6J embryonic stem cells (CRIB6.1, an ES line derived in house) were used.

References

• Original: J:243751 Espana-Agusti J, et al., Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells. PLoS One. 2016;11(2):e0148055
• All: 1 reference(s)