Pkn2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pkn2^{tm1c(KOMP)Wtsi}
• Name: protein kinase N2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5911965
• Synonyms: Pkn2^f
• Gene: Pkn2 Location: Chr3:142496663-142587765 bp, - strand Genetic Position: Chr3, 66.69 cM
• Alliance: Pkn2^{tm1c(KOMP)Wtsi} page
• IMPC: Pkn2 gene page

Mutation origin

• Mutant Cell Line: EPD0319_7_G05
• Germline Transmission: Earliest citation of germline transmission: J:229595
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 142526074 of Chromosome 3 upstream of exon 8 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 8 at position 142526834. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp-mediated recombination. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:229595)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pkn2^{tm1c(KOMP)Wtsi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pkn2 Mutation: 84 strains or lines available

References

• Original: J:229595 Quetier I, et al., Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion. Cell Rep. 2016 Jan 26;14(3):440-8
• All: 4 reference(s)