Tg(CAG-PPARG*,-tdTomato)#Sig
Transgene Detail

Summary

• Symbol: Tg(CAG-PPARG*,-tdTomato)#Sig
• Name: transgene insertion, Curt D Sigmund
• MGI ID: MGI:5912198
• Synonyms: PPARgamma-P467L
• Transgene: Tg(CAG-PPARG*,-tdTomato)#Sig Location: unknown
• Alliance: Tg(CAG-PPARG*,-tdTomato)#Sig page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-PPARG*,-tdTomato)#Sig expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PPARG (5468)

  Pparg

  (MGI:97747)

  Mutated human gene resulting in a Pro467Leu substitution

• Mutation details: The transgenic construct comprised the following elements: a CAG promoter, a loxP site flanked STOP cassette, a splicable intron, a mutant human PPARG coding region (from a cDNA) that codes for a substitution resulting in a Pro467Leu change, an IRES in front of the tdTomato reporter gene, a WPRE transcription enhancer and a polyA signal. The construct was injected into the pronuclei of fertilized C57BL/6 x SJL eggs. Only after cre-mediated recombination (which will remove the STOP cassette), will the human and reporter genes be expressed. The mutation in the human gene results in a dominant negative allele. (J:242422)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:242422 Stump M, et al., Effect of selective expression of dominant-negative PPARgamma in pro-opiomelanocortin neurons on the control of energy balance. Physiol Genomics. 2016 Jul 01;48(7):491-501
• All: 2 reference(s)