Camsap3^tm1.1Ltm
Targeted Allele Detail

Summary

• Symbol: Camsap3^tm1.1Ltm
• Name: calmodulin regulated spectrin-associated protein family, member 3; targeted mutation 1.1, Masatoshi Takeichi
• MGI ID: MGI:5912533
• Synonyms: Camsap3^dc
• Gene: Camsap3 Location: Chr8:3637293-3659075 bp, + strand Genetic Position: Chr8, 1.92 cM, cytoband A1.1
• Alliance: Camsap3^tm1.1Ltm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229814
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site and FRT-flanked neo cassette were inserted in intron 13 and a loxP site was inserted in intron 17 via homologous recombination. Cre-mediated recombination removed the neo cassette and exons 14-17. RT-PCR confirmed the expression of a truncated transcript lacking the CKK domain essential for the binding to microtubules. In polarized epithelial cells the mutant protien fails to collect in the apical cortical area of cell-cell junctions. (J:229814)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Camsap3 Mutation: 65 strains or lines available

References

• Original: J:229814 Toya M, et al., CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):332-7
• All: 3 reference(s)