Mrgprd^{tm1.1(cre/ERT2)Wql}
Targeted Allele Detail

Summary

• Symbol: Mrgprd^{tm1.1(cre/ERT2)Wql}
• Name: MAS-related GPR, member D; targeted mutation 1.1, Wenqin Luo
• MGI ID: MGI:5924939
• Gene: Mrgprd Location: Chr7:144868572-144877796 bp, + strand Genetic Position: Chr7, 89.06 cM
• Alliance: Mrgprd^{tm1.1(cre/ERT2)Wql} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:245897
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Mrgprd^{tm1.1(cre/ERT2)Wql} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mrgprd (MGI:3033142)

• Mutation details: A targeting construct was designed to insert a creERT2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain) followed by a frt-flanked neomycin resistance (neo) cassette, just downstream of the start codon of the MAS-related GPR, member D (Mrgprd) gene. Breeding to B6.129S4-Gt(ROSA)26Sor/J (Stock No. 007844) removed the neo cassette. (J:245897)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mrgprd (mouse) Summary of all recombinase alleles driven by Mrgprd. Comparative matrix view of recombinase activities Alliance mouse Mrgprd gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mrgprd Mutation: 26 strains or lines available

References

• Original: J:245897 Olson W, et al., Sparse genetic tracing reveals regionally specific functional organization of mammalian nociceptors. Elife. 2017 Oct 12;6:e29507
• All: 18 reference(s)