Cacna2d4^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cacna2d4^em1(IMPC)J
• Name: calcium channel, voltage-dependent, alpha 2/delta subunit 4; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:5925303
• Gene: Cacna2d4 Location: Chr6:119213487-119329368 bp, + strand Genetic Position: Chr6, 56.62 cM
• Alliance: Cacna2d4^em1(IMPC)J page
• IMPC: Cacna2d4 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences ATCAGATGTCAGATCCTCAA, TCTGACATCTGATCGTGATA, GCAGGGAGACTCTACTTACA and GGGGCTCAAACACAGTCAGA, which resulted in a 173 bp deletion beginning at Chromosome 6 positive strand position 119,238,969 bp and ending after 119,239,141 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000332836 (exon 2) and 91 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 83 and early truncation 8 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cacna2d4 Mutation: 76 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)