Dot1l^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dot1l^{tm1c(KOMP)Wtsi}
• Name: DOT1 like histone lysine methyltransferase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5925348
• Synonyms: Dot1l^fl
• Gene: Dot1l Location: Chr10:80591040-80631295 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Dot1l^{tm1c(KOMP)Wtsi} page
• IMPC: Dot1l gene page

Mutation origin

• Mutant Cell Line: EPD0018_2_D02
• Germline Transmission: Earliest citation of germline transmission: J:246059
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gtk
• Mutation details: The L1L2_gtk cassette was inserted at position 80599973 of Chromosome 10 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 2 at position 80600773. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving exon 2 flanked by loxP sites. (J:246059)

Expression

In Mice Carrying this Mutation:	4 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dot1l Mutation: 72 strains or lines available

References

• Original: J:246059 Monteagudo S, et al., DOT1L safeguards cartilage homeostasis and protects against osteoarthritis. Nat Commun. 2017 Jun 19;8:15889
• All: 23 reference(s)