Slc9a8^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc9a8^{tm1c(KOMP)Wtsi}
• Name: solute carrier family 9 (sodium/hydrogen exchanger), member 8; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5925353
• Synonyms: Nhe8^lox
• Gene: Slc9a8 Location: Chr2:167263632-167318920 bp, + strand Genetic Position: Chr2, 87.22 cM
• Alliance: Slc9a8^{tm1c(KOMP)Wtsi} page
• IMPC: Slc9a8 gene page

Mutation origin

• Mutant Cell Line: EPD0033_5_A07
• Germline Transmission: Earliest citation of germline transmission: J:245977
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 167282591 of Chromosome 2 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 4 at position 167283331. Exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 4. (J:245977)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc9a8 Mutation: 108 strains or lines available

References

• Original: J:245977 Oberheide K, et al., Loss of the Na+/H+ exchanger NHE8 causes male infertility in mice by disrupting acrosome formation. J Biol Chem. 2017 Jun 30;292(26):10845-10854
• All: 1 reference(s)